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Fig. 6 Target three key gene loci of two key tsRNAs in NSCPO tissues. (a) 5’tiRNA-35-GlyTCC-3 targets BMP6. (b) 5’tiRNA-35-GlyTCC-3 targets CUL1. (c) 5’tiRNA-33-CysGCA-11 targets SPR

Journal: BMC oral health

Article Title: 5'tiRNA-35-GlyTCC-3 and 5'tiRNA-33-CysGCA-11 target BMP6, CUL1 and SPR of non-syndromic cleft palate.

doi: 10.1186/s12903-025-05661-8

Figure Lengend Snippet: Fig. 6 Target three key gene loci of two key tsRNAs in NSCPO tissues. (a) 5’tiRNA-35-GlyTCC-3 targets BMP6. (b) 5’tiRNA-35-GlyTCC-3 targets CUL1. (c) 5’tiRNA-33-CysGCA-11 targets SPR

Article Snippet: Primary antibodies were employed in opposition to the subsequent proteins: BMP6 (M06924-1, Boster, China), SPR (bs-11784R, Bioss Antibodies, China) and CUL1 (K003246P, Solarbio, China).

Techniques:

Fig. 9 Relative expression of three key mRNAs SPR (a), BMP6 (b) and CUL1 (c) in clinical samples by RT-qPCR. (**** p < 0.0001; ***p < 0.001; **p < 0.01)

Journal: BMC oral health

Article Title: 5'tiRNA-35-GlyTCC-3 and 5'tiRNA-33-CysGCA-11 target BMP6, CUL1 and SPR of non-syndromic cleft palate.

doi: 10.1186/s12903-025-05661-8

Figure Lengend Snippet: Fig. 9 Relative expression of three key mRNAs SPR (a), BMP6 (b) and CUL1 (c) in clinical samples by RT-qPCR. (**** p < 0.0001; ***p < 0.001; **p < 0.01)

Article Snippet: Primary antibodies were employed in opposition to the subsequent proteins: BMP6 (M06924-1, Boster, China), SPR (bs-11784R, Bioss Antibodies, China) and CUL1 (K003246P, Solarbio, China).

Techniques: Expressing, Quantitative RT-PCR

Fig. 10 Immunohistochemistry staining of BMP6, CUL1, SPR in the Health, Ctrl and NSCPO tissue

Journal: BMC oral health

Article Title: 5'tiRNA-35-GlyTCC-3 and 5'tiRNA-33-CysGCA-11 target BMP6, CUL1 and SPR of non-syndromic cleft palate.

doi: 10.1186/s12903-025-05661-8

Figure Lengend Snippet: Fig. 10 Immunohistochemistry staining of BMP6, CUL1, SPR in the Health, Ctrl and NSCPO tissue

Article Snippet: Primary antibodies were employed in opposition to the subsequent proteins: BMP6 (M06924-1, Boster, China), SPR (bs-11784R, Bioss Antibodies, China) and CUL1 (K003246P, Solarbio, China).

Techniques: Immunohistochemistry, Staining

The combinations of transfected plasmids

Journal: Journal of Assisted Reproduction and Genetics

Article Title: BMAL1 improves assisted reproductive technology outcomes in patients with polycystic ovary syndrome by targeting BMP6 and regulating ovarian granulosa cell apoptosis

doi: 10.1007/s10815-024-03377-2

Figure Lengend Snippet: The combinations of transfected plasmids

Article Snippet: BMP6 Polyclonal antibody , 55,421–1-AP , Proteintech , 1:1000.

Techniques: Transfection, Plasmid Preparation

Primers used for quantitative real-time polymerase chain reaction

Journal: Journal of Assisted Reproduction and Genetics

Article Title: BMAL1 improves assisted reproductive technology outcomes in patients with polycystic ovary syndrome by targeting BMP6 and regulating ovarian granulosa cell apoptosis

doi: 10.1007/s10815-024-03377-2

Figure Lengend Snippet: Primers used for quantitative real-time polymerase chain reaction

Article Snippet: BMP6 Polyclonal antibody , 55,421–1-AP , Proteintech , 1:1000.

Techniques: Sequencing

Antibody information

Journal: Journal of Assisted Reproduction and Genetics

Article Title: BMAL1 improves assisted reproductive technology outcomes in patients with polycystic ovary syndrome by targeting BMP6 and regulating ovarian granulosa cell apoptosis

doi: 10.1007/s10815-024-03377-2

Figure Lengend Snippet: Antibody information

Article Snippet: BMP6 Polyclonal antibody , 55,421–1-AP , Proteintech , 1:1000.

Techniques:

Comparison of the expression levels of BMAL1 and BMP6 in human OGCs between PCOS and control groups. A, B BMAL1 and BMP6 mRNA levels between PCOS and control groups. C–E BMAL1 and BMP6 protein levels between PCOS and control groups. F Correlations between the mRNA expression levels of BMP6 and BMAL1 in OGCs. * P < 0.05, ** P < 0.01, and *** P < 0.001

Journal: Journal of Assisted Reproduction and Genetics

Article Title: BMAL1 improves assisted reproductive technology outcomes in patients with polycystic ovary syndrome by targeting BMP6 and regulating ovarian granulosa cell apoptosis

doi: 10.1007/s10815-024-03377-2

Figure Lengend Snippet: Comparison of the expression levels of BMAL1 and BMP6 in human OGCs between PCOS and control groups. A, B BMAL1 and BMP6 mRNA levels between PCOS and control groups. C–E BMAL1 and BMP6 protein levels between PCOS and control groups. F Correlations between the mRNA expression levels of BMP6 and BMAL1 in OGCs. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: BMP6 Polyclonal antibody , 55,421–1-AP , Proteintech , 1:1000.

Techniques: Comparison, Expressing, Control

The mRNA levels of BMAL1 and BMP6 in OGCs are correlated with ART outcomes of patients. Spearman’s correlation analyses were used to investigate the correlation between the mRNA levels of BMAL1 and the number of retrieved oocytes ( A ), 2PN fertilized oocytes ( B ), available embryos ( C ), and high-quality embryos ( D ). Spearman’s correlation analyses were used to investigate the correlation between the mRNA levels of BMP6 and the number of retrieved oocytes ( E ), 2PN fertilized oocytes ( F ), available embryos ( G ), and high-quality embryos ( H )

Journal: Journal of Assisted Reproduction and Genetics

Article Title: BMAL1 improves assisted reproductive technology outcomes in patients with polycystic ovary syndrome by targeting BMP6 and regulating ovarian granulosa cell apoptosis

doi: 10.1007/s10815-024-03377-2

Figure Lengend Snippet: The mRNA levels of BMAL1 and BMP6 in OGCs are correlated with ART outcomes of patients. Spearman’s correlation analyses were used to investigate the correlation between the mRNA levels of BMAL1 and the number of retrieved oocytes ( A ), 2PN fertilized oocytes ( B ), available embryos ( C ), and high-quality embryos ( D ). Spearman’s correlation analyses were used to investigate the correlation between the mRNA levels of BMP6 and the number of retrieved oocytes ( E ), 2PN fertilized oocytes ( F ), available embryos ( G ), and high-quality embryos ( H )

Article Snippet: BMP6 Polyclonal antibody , 55,421–1-AP , Proteintech , 1:1000.

Techniques:

BMP6 is a downstream target of BMAL1. A Predicted binding site and mutated binding site of BMAL1 in the promoter of BMP6[created with figdraw.com]. B Direct interaction between BMAL1 and BMP6 detected by the dual-luciferase assay. * P < 0.05, ** P < 0.01, and *** P < 0.001

Journal: Journal of Assisted Reproduction and Genetics

Article Title: BMAL1 improves assisted reproductive technology outcomes in patients with polycystic ovary syndrome by targeting BMP6 and regulating ovarian granulosa cell apoptosis

doi: 10.1007/s10815-024-03377-2

Figure Lengend Snippet: BMP6 is a downstream target of BMAL1. A Predicted binding site and mutated binding site of BMAL1 in the promoter of BMP6[created with figdraw.com]. B Direct interaction between BMAL1 and BMP6 detected by the dual-luciferase assay. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: BMP6 Polyclonal antibody , 55,421–1-AP , Proteintech , 1:1000.

Techniques: Binding Assay, Luciferase

The impact of BMAL1 on the expression of BMP6 in KGN cells. A, B Expression levels of BMAL1 and BMP6 mRNA in KGN cells after transfection. C, D Expression levels of BMP6 protein in KGN cells after transfection. OE, overexpression; NC, negative control. * P < 0.05, ** P < 0.01, and *** P < 0.001

Journal: Journal of Assisted Reproduction and Genetics

Article Title: BMAL1 improves assisted reproductive technology outcomes in patients with polycystic ovary syndrome by targeting BMP6 and regulating ovarian granulosa cell apoptosis

doi: 10.1007/s10815-024-03377-2

Figure Lengend Snippet: The impact of BMAL1 on the expression of BMP6 in KGN cells. A, B Expression levels of BMAL1 and BMP6 mRNA in KGN cells after transfection. C, D Expression levels of BMP6 protein in KGN cells after transfection. OE, overexpression; NC, negative control. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: BMP6 Polyclonal antibody , 55,421–1-AP , Proteintech , 1:1000.

Techniques: Expressing, Transfection, Over Expression, Negative Control